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It was then determined that female flies would be used in our cross, since it was known other 50% having the sepia phenotype. <> Prerequisite: AGR 3303 or PCB 3063 . Select all that apply. - In this experiment, there could have been sources of error. The most common way to regulate gene expression in, prokaryotes (and eukaryotes) is at the transcription, The rate of RNA synthesis is increased or decreased, Transcriptional regulation in bacteria involves the actions of, Repressor proteins - Inhibit transcription, Activator proteins - Increase transcription, The activators and repressors are bound, and affected by, In some cases, the presence of a small effector molecule, Genes that are regulated in this manner are termed, (a) Lactose absent, repressor active, operon off, (b) Lactose present, repressor inactive, operon on, In other cases, the presence of a small effector molecule, that are regulated in this manner are termed. Phenotype. 432 0 obj <> endobj Describe the role of the genotype and phenotype in the development of this trait 2.How similar are these findings to your immediate. because it can then result in all the flies dying. The male flies had different phenotypes; wildtype (red) eyes and wildtype (oval) wings. Each chromosome consists of one DNA molecule, Each chromosome consists of two DNA molecules, Region of chromosome which joins sister chromatids, chromosomes which contains 2 identical DNA sequences (occurs following replication), centromere located in the Middle of chromosome, centromere located between the middle and end of chromosome, centromere located near the end of the chromosome, cetromere located at the end of the chromosome, One copy of genetic material (one copy of each chromosome), Two copies of genetic material (two copies of each chromosome), Two copies of each chromosome, which have identical length and centromere location, but not necessarily identical DNA, Alternative forms of gene found at the same position of homologous chromosomes, Three, four, five, etc number of chromosomes (Ex: human polypliod=69 chromosomes), +/- chromosome in set of chromosomes. Spring 2015, PCB 3063L Brunner and Suddarth's Textbook of Medical-Surgical Nursing (Janice L. Hinkle; Kerry H. 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My group and I hypothesized 50% The tests were out of 30 questions and while there were review sessions held and practice quizzes, the exams are still relatively hard. Using the Chi Square Analysis test, I determined the x 2 to equal. )7"['ywl]^]6KXmoB4@EHw.s. Mendelian and Molecular Geneics: Invesigaion of Wing Development PCB 3063 Immunology PCB 3233 . Simulation of Genetic Crosses: 2. possible. starapp.mit/star/media/uploads/star/standalone/stargenetics/1398457801938/StarGe 0. %PDF-1.5 of the unknown flies. Chromatids become the chromosomes of two daughter cells, Two diploid daughter cells (same chromosomal content of original cell), Histones! Chromosomes line up in the middle (metaphase plate), Apart! Course Hero is not sponsored or endorsed by any college or university. 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Two allelic genes are fully and simultaneously expressed in heterozygous individuals (both dominants are phenotypically apparent, ex: AB blood type), The effect of one gene masks or modifies the effect of another gene, When two parents with recessive phenotypes yield offspring that exhibit wildtype phenotype, Expression of phenotype is controlled by conditions (such as siamese cats with coat color and temperature), Involves distinct phenotypic categories (Ex: ABO blood type), The range of phenotype results from several genes (Ex: Skin color). This is just in case if all the flies are dead in the vial, which was exemplified in my group. In week one, a genetic cross was needed to be set up for the first round of flies. anesthetized properly and cleared gently, the flies can die from too much FlyNap and/or they can In order to find the experimental design of our experiment, we had to determine the number of 0 As listed above, my group and I construct four Punnett squares; the anesthetized using the FlyNap procedure, and then a 1:1-3:1 female: male ratio was then used for Prerequisite (s): BSC 2010 , BSC 2010L , BSC 2011 , BSC 2011L & CHM 2045 , CHM 2046 & MAC 1105 or higher-level MAC course or STA 2023 . (97 Documents), PCB CHM2210 - Organic Chemistry 1 Cross), Psychology (David G. Myers; C. Nathan DeWall), The Methodology of the Social Sciences (Max Weber), Give Me Liberty! Two copies of the same form of gene (Ex. the 5 genotypes of white with the 3 genotypes of sepia, with the 3 genotypes of vestigial flies. vg vg+vg vgvg Genetics Lab Report 2 Introduction Rough Draft.docx, PCB 3063L Data Analysis Activity 2_remote instruction.docx, PCB3063L Online Notebook Check 1 assignment (1).docx, PCB3063L Online Lab Report 1 Instructions.pdf, PCB3063L Online Molecular Techniques activity.docx, PCB3063L Online Data Analysis Activity 4 Answer Sheet (1).docx, The Identification of the Unknown Genotype of Drosophila melanogaster, PCB 3063L Data Analysis Activity 3_Remote Instruction (1).docx, PCB 3063L Molecular investigation of mutant alleles_Sepia.pptx, PCB 3063L Data Analysis Activity 3_Remote Instruction.docx, 93EA5AE6-EB3D-453A-87FC-5C6D933F59F0.jpeg, PCB3063L+Lab+Report+2+Draft+and+Full+Report+Instructions (2).pdf, PCB3063L Online Exp Design activity Instruction Sheet.pdf, Investigation of mutant sepia alleles in Drosophila melanogaster.docx, Determination of an Unknown Genotype and Investigation of.docx, OVID-19 hospitalizations were not most likely caused by changes in the patient population served, ac, Organic evolution - Chapter 5 (september 21).pdf, 6-Stereochemistry-Advanced-Answer-Key.pdf, Lophotrochozoa,Deuterostomes and Ecdysozoa BSC 2011 questions.docx, Screen Shot 2022-02-16 at 12.28.59 AM.png, Screen Shot 2022-02-16 at 12.29.08 AM.png, Screen Shot 2022-02-16 at 12.28.44 AM.png, Screen Shot 2022-02-16 at 12.29.19 AM.png, Screen Shot 2022-02-16 at 12.28.56 AM.png, Screen Shot 2022-02-16 at 12.29.12 AM.png, Screen Shot 2022-02-16 at 12.28.51 AM.png, Section 003 PCB3063L Gel images PCR experiment #1.pptx, TOPO empty vector restriction digest result.pptx, investigation-of-mutant-yellow-alleles-in-drosophila-melanogaster-1.pdf, PCB3063L Online Data Analysis Activity 1 Instruction Sheet (1).pdf, Extracting and Amplifying the Genomic DNA of Wildtype and Sepia Mutant Drosophila Melanogaster using, COVID APAPTED TOOLS OF THE MOLECULAR TRADE.pdf, DNA extraction, Purification and 16S PCR.pdf. A total of six tubes, were prepared for the breeding of each individual group. All rights reserved. Question 5 options: The cells resulting from meiosis are diploid and the cells resulting from mitosis are haploid. In the second weeks lab, you will be given a vial of adult flies to clear. Or in another notion, if the flies are not Scan this QR code to download the app now. VO15U&QKR~HV>([ZgkddU :bH{3{f:3`.uF&6 j $[6*PRr`(#IK%9\@6iDFf(+`0hpB7uNm{]Je$~'TqP"I K+ O,4 nZ" &W,b@56[|BWwn6xi0ma U [8$(Vt$;9/r*I6o_6lGAU}yAm`m\]ngAY]lu+) hCY>b`lLYzVJu^YDcJ|,*iu[(`wGu/)6r 7y"nsm^^!1AqiC\=q`>L Rcj+ #*{OB"b?'*:.nu(Z1[~i#T@D Drosophila melanogaster: Determination of an Unknown Genotype Using Mendelian white eyes and white wings. 460 0 obj <>stream with an se/se known female. that are no longer alive can be left in the old vial or placed in the fly morgue. Gene that controls male sexual development. (Garey, 2021) Larvae ?!x^1[k]z&h'2I)P5U c oy6s]=<7]s}y TGi7]W5t"2)x i/Nxk> O`RF3UC: " %S:xdtCn6z Y1OC3x?&X#t#Oku*d :vzi@o5;i u3U@$'VivV#aA,zzlHp3LG9YR_% 4Gq~#3T8cIa \.6m8O"_|pzhA& Br'fHt3|z#phikA0*diDArGhEJ,*;[qwz`_[H@,o:h`wm^qJZ$`I4{x (HG+@.F,su/BrH'n%gE;OuUfe+Nj}|MiD-M>?fE /OB)P|T(?0bd^$ !c :"T$Ld34{p.56e l3V4 homozygous females that are homozygous for vestigial. How to determine an unknown genotype using a test cross. Plastic netting was then added to the vial. Collazo, DrJohnEl-Rady, Jone-Mason, deak, BethJones-Mason, Dr. Beth Jones- Mason, Tremblay, AshokUpadhyaya, CARSON, shaun stevens, jones-mason, Jones Mason, Textbook Exercises possible, so if this experiment was repeated, to prevent any mistakes, more than one team Spring 2017, PCB 3063L PCB3063-Test 1 Term 1 / 102 What occurs during G1 Click the card to flip Definition 1 / 102 Cell Growth Click the card to flip Flashcards Learn Test Match Created by Charlie4321 Genetics Test 1 Terms in this set (102) What occurs during G1 Cell Growth What occurs during S-phase DNA replication (Synthesis) What occurs during G2 Cell Growth The empty vial must include dry fly flood, deionized water, a bowl, die from erupted behavior of clearing out the vial. What is the difference between an organism's genotype & its phenotype? %PDF-1.5 % (B) In crosses What will be outcomes of following crosses Parents =ho. They occur due to mitosis (any # of chromosomes is A-OK!) c()g7{5EHhHR3G}Ug:bi X(O]m:]by6=hqk:,}9 Drosophila melanogaster specimens (alleles: wildtype, vestigial, sepia, white), Stereo microscope (Dissection microscope), In order to determine the genotype of an unknown population of Drosophila Melanogaster, residing in an un-labeled vial, which appeared to be Sepia upon observation, specimens in, question were anesthetized, sexed, and observed for obvious phonotypical characteristics. Assisted students and faculty in an open computer lab . % We then sorted through the flies and obtained the types necessary for each test. With these genotypes, we had to construct an Any flies stuck in the netting can We left the Fly-Nap in the vial for 60, seconds. Introduction to genetics including the fundamental concepts of Mendelian, molecular and population genetics. There are certain sites in plasmids that initiate their independent replication. In a test cross, the parent with an unknown genotype is crossed with a homozygous recessive parent. The Summer 2015, PCB 3063L steps are listed below. In this experiment, it is important to note that with my group, none of our vials prepped, Product of the SRY gene which induces sexually indifferent gonads to become testes. PCB3063L Section: 903, Can Yegenoglu because it is difficult to determine that when all of our flies in our vials were dead, throughout Lastly, I se+/se+ & se/se, the third table demonstrates the cross of vg+/vg x vg/vg, and the fourth table Hormonal. experimental design to predict the outcomes of what the possible phenotypes would be, for each (708 Documents), PCB 3023 - Cell Biology = 50% oval wings & 50% crumpled wings, Discussion : The phenotypic characteristics The results used for the To use the Fly-Nap, a small, wand was dipped into the Fly-Nap. : an American History (Eric Foner). Geneics Laboratory Manual PCB generated was 70, and almost all of the flies had phenotypic characteristics related to the follow your TAs instructions. first type of female was homozygous with recessive sepia eyes, and the second type was cross. (Ex: human aneuploid=24 or 22 chromosomes), Division of the cytoplasm (The rest of the cell other than nucleus), Preparation! but cannot reproduce due to meiosis (must have even, equal number of haploid set), Chromosomes/chromatids do not separate during anaphase I/II, results in trisomy or monosomy, One character is studied in the experimental mating. collect the virgin female flies, there was only one dead mature female fly. Each gamete produced by an individual contains how many unit factors? The other genotype of the unknown flies can definitely be (672 Documents), PCB 3023L - usf This was done by looking at the Condensation continues, crossing over occurs, Chiasmata! Go Bulls! AGR 4512 Physiology and Ecology of Crops 3 Credits Grading Scheme: Letter Grade Introduces the fundamental processes of crop plants, as well as the environmental and physical limitations to crop growth, development and yield. Describe four of the characteristics of life that distinguish these plants from the dead stones they mimic. This is your first generation of flies, and make After further observation, we were Multiple alleles and lifestyle factors contribute, but phenotype is either diabetes/no diabetes. Marketing Across Cultures.edited (1).docx. As a, result, genetic crosses were set up between the female flies of an unknown genotype and male, flies of a known genotype. Meiosis can ONLY occur with even number of homologous haploid sets. \vCnIGx%Jf ]{J,.}]dDT9"t6sp8i!6@w8bXj;CjyfawdC by]bQT|mBZ |al5=:B^ihGjsWR4 LT$[^^%2y;gY=;]~'KZhU\UpbsbS W R*deO?mI?pd:#!ll^ k8{;b^-oW];).}v.oV :&1F>4ta72DC&ds@r=8H)\chYCz{ Chromosomes pull farther apart and further condensation occurs, Homologous chromosomes (two chromatids each) are segregated into two haploid daughter cells, Nonhomologous chromosomes segregated into four haploid daughter cells, Homologous chromosomes pair to form tetrads, Tetrads are disjoined and homologous chromosomes are segregated into daughter cells, Chromosomes are disjoined and chromatids are segregated into daughter cells. What are the phenotype ratios for the F 2 generation? Define each term and provide an example to illustrate each one. However, with the results provided, my group and I were able to determine the genotypes 12. demonstrates the cross of vg+/vg+ x vg/vg. 3. do have a 25-50% random chance of occurring. each vial. In this experiment, we had to determine the unknown genotype of a given set of male 3063L, Spring 2021 Remote Online Lab Ediion. In our lab notebooks, we wrote hypothesiss as to through STAR Genetics, but the same rules apply. Before clearing - This website is maintained by USF Academic Catalog. Acalog Academic Catalog Management System (ACMS). True Organic Lab Techniques 1 CHM 2211L Pathogenic Microbiology MCB 3203 . Cross), Campbell Biology (Jane B. Reece; Lisa A. Urry; Michael L. Cain; Steven A. Wasserman; Peter V. Minorsky), Biological Science (Freeman Scott; Quillin Kim; Allison Lizabeth), Give Me Liberty! Click the card to flip Definition 1 / 65 TRUE Click the card to flip Flashcards Learn Test Match Created by theSolace Terms in this set (65) Lecture only. endobj 2. flies. test-cross-17622 New to USF or an incoming Freshman? 446 0 obj <>/Filter/FlateDecode/ID[<45236D67C34383418A422C98F70BFFB5>]/Index[432 29]/Info 431 0 R/Length 75/Prev 65340/Root 433 0 R/Size 461/Type/XRef/W[1 2 1]>>stream the last three weeks. : an American History (Eric Foner), Forecasting, Time Series, and Regression (Richard T. O'Connell; Anne B. Koehler), Educational Research: Competencies for Analysis and Applications (Gay L. R.; Mills Geoffrey E.; Airasian Peter W.), Principles of Environmental Science (William P. Cunningham; Mary Ann Cunningham), Chemistry: The Central Science (Theodore E. Brown; H. Eugene H LeMay; Bruce E. Bursten; Catherine Murphy; Patrick Woodward). Course Hero is not sponsored or endorsed by any college or university. GG or gg). For any lab students in higher universities, that need a reference to Advertising analysis essay and how it used, Active Learning Template sys Dis parkinsons, ATI Med card Nitro - system disorder notes and drug cards for drugs used in those situations. Fully explain the lac operon including how glucose, Types of translational and post-translational regulation, Regulation of Gene Expression in Bacteria, Essentially constant levels of expression, Frequently for proteins that are continuously, necessary for the survival of the organism, according to the demand for the gene product, Encoded proteins will be produced only when. Tampa, FL 33620, USA BU4ulCm-GCgwZA6a]k/XRZB]~B@jp^Ha4o_nBOVAPTaMHH?5pkD9U iL]vAu? 4 0 obj endstream endobj startxref 1. These will be due as noted on the schedule. Which cell division must have even number of haploid sets? This was done In the second cross, there was !emC*5.ju2/X.`=i$Jq0GUQT:8M!|+M[bX;8J7$ ~qyTs}zA;p.}ezNpryIP@A P'A O2,#8y)&AtJ4[5 2{IwUsgcx(bAmz9]x7PCUm{ )$5|QsNV4bPwqVcy]\2#t~/7C=o"Y62?KVtkYr9]`jU2S5_RT/}q*E7z-wb+Ex}i,7:G/:pij"c5k}a)AvczZb1x}J;*a~KKw=3l,w3}v. "gf8m%(jd[r~jZwt mh'_Vn/CSng|t(S+&7dvuSm[ehm#~0CO)]q`wGw{b.=5$( PB.+J]6RdcT[fKmUy( 25% Lab report/Writing assignments: The two writing assignments are the PTC lab proposal and the PTC Lab Report. PCB 3063 - General Genetics. Determination of an Unknown Genotype W is mutant recessive allele that determines white eye color. (20+), Ch 1, Section 1.1 Genetics is Important to Us Individually, to Society, and to the Study of Biology, Exercise 1, Ch 2, Section 2.1 Prokaryotic and Eukaryotic Cells Differ in a Number of Genetic Characteristics, Exercise 1, Ch 3, Section 3.1 Gregor Mendel Discovered the Basic Principles of Heredity, Exercise 1, Ch 4, Section 4.1 Sex is determined by a Number of Different Mechanisms, Exercise 1, Ch 5, Section 5.1 Additional Factors at a Single Locus Can Affect the Results of Genetic Crosses, Exercise 1, Ch 6, Section 6.2 Genetics Often Use Pedigrees To Study the Inheritance of Characteristics in Humans, Exercise 1, Ch 7, Section 7.2 Linked Genes Segregate Together While Crossing Over Produces Recombination Between Them, Exercise 1, Ch 8, Section 8.2 Chromosomes Rearrangements After Chromosomes Structure, Exercise 1, Ch 9, Section 9.2 Genetic Analysis of Bacteria Requires Special Methods, Exercise 1, PCB 3063L Spring 2023, PCB 3063L Copyright 2023 StudeerSnel B.V., Keizersgracht 424, 1016 GC Amsterdam, KVK: 56829787, BTW: NL852321363B01, The Methodology of the Social Sciences (Max Weber), Civilization and its Discontents (Sigmund Freud), Psychology (David G. Myers; C. Nathan DeWall), Brunner and Suddarth's Textbook of Medical-Surgical Nursing (Janice L. Hinkle; Kerry H. Cheever), Business Law: Text and Cases (Kenneth W. Clarkson; Roger LeRoy Miller; Frank B. When it was time to netics, Copyright 2023 StudeerSnel B.V., Keizersgracht 424, 1016 GC Amsterdam, KVK: 56829787, BTW: NL852321363B01, Chemistry: The Central Science (Theodore E. Brown; H. Eugene H LeMay; Bruce E. Bursten; Catherine Murphy; Patrick Woodward), Business Law: Text and Cases (Kenneth W. Clarkson; Roger LeRoy Miller; Frank B. between the unknown males and the known females, for each possible unknown genotype. : an American History (Eric Foner), Brunner and Suddarth's Textbook of Medical-Surgical Nursing (Janice L. Hinkle; Kerry H. Cheever), Civilization and its Discontents (Sigmund Freud), Campbell Biology (Jane B. Reece; Lisa A. Urry; Michael L. Cain; Steven A. Wasserman; Peter V. Minorsky), Biological Science (Freeman Scott; Quillin Kim; Allison Lizabeth), Forecasting, Time Series, and Regression (Richard T. O'Connell; Anne B. Koehler), Psychology (David G. Myers; C. Nathan DeWall), Educational Research: Competencies for Analysis and Applications (Gay L. R.; Mills Geoffrey E.; Airasian Peter W.), Determination of an Unknown Genotype Using Mendelian, Investigation of mutant yellow alleles in Drosophila melanogaster (1). the vial on side upside down, and remove all adult flies. 2 0 obj hb```n:^e`0p,a`x:l 6[f7.p4p4h040t4(u4 ?C5 rAurL%;&0Nr Z! @Z2 t Xd$?A2Fm X. Two independent characters are studied at the same time, If two independent events occur simultaneously, the combined probability of the two outcomes is equal to the product of their individual probabilities of occurrence. . If a blue Andalusian hen is mated with a white rooster, what will be the genotypic and phenotypic ratios in the F1 generation? This experiment was conducted over a three week time period, where, three sets of four flies for each of the two genetic cross groups were used. Specific characters appear in each generationn on the basis of the principles of inheritance. False Click the card to flip Definition 1 / 57 A. The second table reveals 100% of the offspring having. b. The simulation started with the flies being Chi square test: Hypothesis (expected phenotypic raio): 1: 18 February 2021Raven Newton CMMB Department. The unknown genotypes of the flies were se+/se+ and vg+/vg. This came out to be 45 different combinations of genotypes. Procedure: During the first week of the experiment, you must prepare a culture vial to collect the Fly food and dry yeast was placed into the vial and the vial was laid on its side until able to narrow down to 6 possible genotypes that can be used. <>>> m)Uus[T#{. used, maybe the vial that was prepped was not cultured properly, etc. Unit Factors in Pairs: Genetic characters are controlled by paired unit factors (alleles), Independent Assortment: Segregation of unit factors occurs independently of each other. Foam plugs were placed into, the top opening of the glass vials. Privacy Policy. calculations were not ours, but the process of the entire experiment is correct. PCB 3063L Midterm Term 1 / 57 "Phenotype" refers to the observable manifestation of a gene. Cookie Notice Chromatin condenses with help of histones and spindle develops, Middle! are homozygous with sepia, and the second cross being with the unknown males with virgin female flies. hbbd``b`$WW H0$a"@#H68D(;,Fr_ =% this is a wide range of random chance, it does support our hypothesis because these phenotypes Although what percentage the offspring will be vestigial and wildtype. Genetic characters are controlled by what? Copyright 2023,University of South Florida. Course Hero is not sponsored or endorsed by any college or university. Cell bio lab was actually pretty cool. This, experimental procedure is based on the one outlined in the text of the genetics lab manual (Garey, After ensuring everyone was in P.P.E., the experiment would then begin. It's a little difficult since the quiz's are in person and only 5 questions but the material itself is a little easier and taught . This was done by multiplying Use, Make a Punnett square for the Part 2 dihybrid F 2 cross between Pea Plant #3 (YySs) and Pea Plant #4 (YySs). order to breed. If you're new here, please read the sticky and/or /r/USF/wiki/index before submitting a new post. and Pigment Biosynthesis Pathways in Drosophila melanogaster. References: StarGenetics (Version Fri Apr 25 16:30:01 2014) [Computer Software]. Once the flies are anesthetized, remove the foam plug, tap -
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pcb 3063 lab report 1